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Rattus norvegicus
30 Downloadable Samples
Affymetrix Rat Expression 230A Array (rae230a), Affymetrix Rat Genome 230 2.0 Array (rat2302)
Description
Gene expression profiling of the medial (MGE), lateral (LGE) and caudal (CGE) ganglionic eminence, and cerebral cortex (CTX) at various embryonic stages (E12.5, E14 and E16).
Publication Title
Comprehensive spatiotemporal transcriptomic analyses of the ganglionic eminences demonstrate the uniqueness of its caudal subdivision.
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 4 Downloadable Samples
Illumina HiSeq 4000
Description
iPSC derived from a patient heterozygous for the SOD1 E100G mutation were genome edited to homozygous wild type using the CRISPR-Cas9 system. Both disease and isogenic corrected iPSC were differentiated into spinal motor neurons that were ISL1+ and CHAT+. Gene expression changes in MN were analyzed by RNA-sequencing.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line
View Samples- Mus musculus
- 22 Downloadable Samples
- Illumina HiSeq 2500
Description
The dual leucine zipper kinase (DLK) is a mitogen-activated kinase kinase kinase that can activate the downstream cJun N terminal kinase (JNK) pathway (ref). We have previously reported that DLK is a positive regulator of the retrograde injury signaling and axon regeneration that unfolds after sciatic nerve injury (ref). Since DLK is required for activities of injury-associated transcription factors such as cJun and STAT3, we hypothesized that DLK is also necessary for the transcriptional responses to peripheral nerve injury. In the current study, we identify DLK-dependent transcriptome in dorsal root ganglion (DRG) neurons using a sciatic nerve injury paradigm. The DEG analysis reveals that DLK regulates regeneration/injury-associated genes in both basal and injured conditions. By performing gene ontology analysis, we suggest functional annotations and the involved genes as regulatory components of the axonal regeneration program. Finally, our comparative analysis indicates that DLK is required for a specific retrograde signaling pathway that regulates a regeneration program shared between PNS and CNS models.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Mus musculus
- 14 Downloadable Samples
- NextSeq 500
Description
we have compared the injury-induced transcriptomic changes between the regenerating proximal segment and the degenerating distal segment of a transected nerve, at different post-injury time points
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Mus musculus
- 4 Downloadable Samples
- Illumina HiSeq 2500
Description
Functional anaysis of mouse Tet genes
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line, Treatment
View Samples
SRP095659- Mus musculus
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
We investigate the molecular mechanisms of the RP9 mutation causing photoreceptor degeneration using the 661w cell
Publication Title
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9.
Sample Metadata Fields
Sex, Specimen part
View Samples SRP095666- Mus musculus
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
RP9 point mutation of 661w cell
Publication Title
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9.
Sample Metadata Fields
Sex, Specimen part
View Samples SRP095667- Mus musculus
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
RP9 konck out of 661w cell
Publication Title
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9.
Sample Metadata Fields
Sex, Specimen part
View Samples- Saccharomyces cerevisiae
- 18 Downloadable Samples
- Affymetrix Yeast Genome S98 Array (ygs98)
Description
Global restriction of protein synthesis is a hallmark of cellular stress. Using hydrogen peroxide, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to separate highly translated and poorly translated mRNAs that were then separately analysed.
Publication Title
Global translational responses to oxidative stress impact upon multiple levels of protein synthesis.
Sample Metadata Fields
Sex, Compound
View Samples- Saccharomyces cerevisiae
- 12 Downloadable Samples
- Affymetrix Yeast Genome S98 Array (ygs98)
Description
One common form of translational control is mediated by proteins that bind to the mRNA 5' cap-binding protein eIF4E. These proteins are collectively called 4E binding proteins (4EBPs). Saccharomyces cerevisiae possesses two 4EBPs that are encoded by non-essential genes called CAF20 and EAP1. To determine the impact of gene deletion on gene expression, we monitored the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes in wild-type, caf20 and eap1 cells. Polyribosome fractionation of cell extracts was used to separate highly translated and poorly translated mRNAs that were then separately analyzed.
Publication Title
Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins.
Sample Metadata Fields
Sex
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