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Accession IconE-MEXP-526

Transcription profiling by array of Saccharomyces cerevisiae after treatment with hydrogen peroxide

Organism Icon Saccharomyces cerevisiae
Sample Icon 18 Downloadable Samples
Technology Badge Icon Affymetrix Yeast Genome S98 Array (ygs98)

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Description
Global restriction of protein synthesis is a hallmark of cellular stress. Using hydrogen peroxide, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to separate highly translated and poorly translated mRNAs that were then separately analysed.
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