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Gallus gallus
28 Downloadable Samples
Affymetrix Chicken Genome Array (chicken)
Description
Global gene expression profiling of the avian B-lymphoma DT40 cell line was used as a model to differentiate among Btk KO and Btk KO cells reconstituted with human Btk. Differences in the gene expression pattern showed statistically significant changes between parental DT40 and all the Btk KO cell populations irrespective of whether they are reconstituted or not. These results imply that in the process of generating a knockout cell line, subclones are selected, which have multiple changes in their gene expression pattern (p<0.01).
Publication Title
Expression profiling of chicken DT40 lymphoma cells indicates clonal selection of knockout and gene reconstituted cells.
Sample Metadata Fields
No sample metadata fields
View Samples- Drosophila melanogaster
- 33 Downloadable Samples
Affymetrix Drosophila Genome 2.0 Array (drosophila2), Affymetrix Drosophila Genome Array (drosgenome1)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.
Sample Metadata Fields
No sample metadata fields
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GSE7646- Drosophila melanogaster
- 4 Downloadable Samples
- Affymetrix Drosophila Genome Array (drosgenome1)
Description
CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line
Publication Title
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.
Sample Metadata Fields
No sample metadata fields
View Samples GSE7652- Drosophila melanogaster
- 10 Downloadable Samples
- Affymetrix Drosophila Genome 2.0 Array (drosophila2)
Description
6 Timepoint microarray from control strain
Publication Title
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.
Sample Metadata Fields
No sample metadata fields
View Samples GSE7651- Drosophila melanogaster
- 9 Downloadable Samples
- Affymetrix Drosophila Genome 2.0 Array (drosophila2)
Description
6 Timepoints from 5073 strain
Publication Title
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.
Sample Metadata Fields
No sample metadata fields
View Samples GSE7644- Drosophila melanogaster
- 4 Downloadable Samples
- Affymetrix Drosophila Genome Array (drosgenome1)
Description
Experiments performed in S2 cells to identify direct CLK targets
Publication Title
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.
Sample Metadata Fields
No sample metadata fields
View Samples- Drosophila melanogaster
- 6 Downloadable Samples
- Affymetrix Drosophila Genome 2.0 Array (drosophila2)
Description
S2 cells transfected with pAc-Clk or empty vector
Publication Title
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 4 Downloadable Samples
Illumina HiSeq 2000
Description
RNAseq characterization of gene expression changes 72 hours after genomic excision of Cebpa in murine hematopoietic progenitors from Cebpaf/f;CreER mice transformed by Hoxa9/Meis1. In the presence of tamoxifen (4OHT), Cre-ER localizes to the nucleus of cells allowing for excision of Cebpa and loss of C/EBPa protein levels. Loss of C/EBPa leads to a decrease in cellular proliferation. Overall design: Examination of gene expression by RNAseq in two conditions in biological replicates.
Publication Title
C/EBPα is an essential collaborator in Hoxa9/Meis1-mediated leukemogenesis.
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
Characterization of gene expression changes 72 hours after withdrawal of tamoxifen in murine hematopoietic progenitors transformed by Hoxa9-ER/Meis1 using RNAseq. In the presence of tamoxifen (4OHT), Hoxa9-ER localizes to the nucleus of cells allowing for transformation, while withdrawal of 4OHT (culture in EtOH) leads to loss of nuclear Hoxa9-ER. Loss of Hoxa9-ER leads to a decrease in cellular proliferation and differentiation along the myeloid lineage. Overall design: Examination of gene expression by RNAseq in two conditions in biological replicates.
Publication Title
C/EBPα is an essential collaborator in Hoxa9/Meis1-mediated leukemogenesis.
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 41 Downloadable Samples
- Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
The anti-HIV humoral immune response following acute infection is delayed and ineffective. HIV envelope protein gp120 binds to and signals through 47 on T cells. We show that gp120 also binds and signals through 47 on B cells, resulting in an abortive proliferative response. In primary B cells, gp120 signaling through 47 resulted in increased expression of TGF-1 and the B cell inhibitory receptor FcRL4. Co-culture of B cells with HIV-infected autologous CD4+ T cells also resulted in increased B cell FcRL4 expression. These findings indicate that, in addition to inducing chronic immune activation, viral proteins can contribute directly to HIV-associated B cell dysfunction, thus providing a mechanism whereby the virus subverts the early HIV-specific humoral immune response.
Publication Title
The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression.
Sample Metadata Fields
Specimen part, Disease, Time
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