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GSE45220
Homo sapiens
9 Downloadable Samples
Affymetrix Human Gene 1.1 ST Array (hugene11st)
Description
Gene(s) in epithelial cells can be upregulated or downregulated by different stimuli in a tissue-specific manner. We used genome-wide microarray analysis to profile the expression of genes in HT-29 cells that are upregulated after stimulation with sodium butyrate (NaB) and subsequently downregulated by the addition of ciprofloxacin antibiotic. The specific aim was to evaluate genes that are associated with mucosal immunity.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Cell line, Treatment
View Samples- Glycine max
- 2 Downloadable Samples
Illumina HiSeq 2000
Description
No description.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Pseudomonas aeruginosa pao1
- 2 Downloadable Samples
- Affymetrix Pseudomonas aeruginosa Array (paeg1a)
Description
Differential expression of regulatory genes of reference strain P. aeruginosa PA01, involved in the quorum sensing was analyzed using Microarray. Total RNA was isolated from reference strain P. aeruginosa PA01, grown with or without bacterial extracts (1.0 mg/ml) using TRI reagent. Total RNA was quantified, and 10 µg RNA was converted to cDNA, fragmented and labelled by following GeneChip P. aeruginosa PA01 genome array user manual. Labelled cDNAs were hybridized with P. aeruginosa genome array gene chip, washed and stained. Hybridized chips were scanned, processed and analyzed using expression console and transcriptome analysis console.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples