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Homo sapiens
298 Downloadable Samples
Affymetrix Human Genome U219 Array (hgu219)
Description
Skin is the largest organ in the body and serves important barrier, regulatory, and sensory functions. Like other tissues, skin is subject to temporal fluctuations in physiological responses under both homeostatic and stressed states. To gain insight into these fluctuations, we investigated the role of the circadian clock in the transcriptional regulation of human epidermal samples collected in a time-ordered fashion. We also determined whether this circadian patterning could be applied to unordered (i.e., randomly collected) human epidermal samples. The purpose of this study was to gain insight into the evolutionarily-conserved rhythmic patterns of the circadian transcriptome in human skin and how it relates to published transcriptomes from other human tissues.
Publication Title
Population-level rhythms in human skin with implications for circadian medicine.
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 505 Downloadable Samples
Affymetrix Human Genome U219 Array (hgu219)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 269 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
Skin is the largest organ in the body and serves important barrier, regulatory, and sensory functions. Like other tissues, skin is subject to temporal fluctuations in physiological responses under both homeostatic and stressed states. To gain insight into these fluctuations, we investigated the role of the circadian clock in the transcriptional regulation of skin
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 236 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
Skin is the largest organ in the body and serves important barrier, regulatory, and sensory functions. Like other tissues, skin is subject to temporal fluctuations in physiological responses under both homeostatic and stressed states. To gain insight into these fluctuations, we investigated the role of the circadian clock in the transcriptional regulation of skin
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 363 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
This study provides an evaluation of changes in gene expression associated with treating human Ishikawa cells with 34 different chemical compounds.
Publication Title
Grouping 34 Chemicals Based on Mode of Action Using Connectivity Mapping.
Sample Metadata Fields
Sex, Cell line
View Samples- Homo sapiens
- 360 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
This study provides an evaluation of changes in gene expression associated with treating human MCF7 cells with 34 different chemical compounds.
Publication Title
Grouping 34 Chemicals Based on Mode of Action Using Connectivity Mapping.
Sample Metadata Fields
Sex, Cell line
View Samples- Homo sapiens
- 348 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
This study provides an evaluation of changes in gene expression associated with treating human HEPG2 cells with 34 different chemical compounds.
Publication Title
Grouping 34 Chemicals Based on Mode of Action Using Connectivity Mapping.
Sample Metadata Fields
Sex, Cell line
View Samples- Homo sapiens
- 331 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
This study provides an evaluation of changes in gene expression associated with treating human HepaRG cells with 34 different chemical compounds.
Publication Title
Grouping 34 Chemicals Based on Mode of Action Using Connectivity Mapping.
Sample Metadata Fields
Sex, Cell line
View Samples
GSE40348- Rattus norvegicus
- 300 Downloadable Samples
- Affymetrix Rat Genome 230 2.0 Array (rat2302)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
A novel transcriptomics based in vitro method to compare and predict hepatotoxicity based on mode of action.
Sample Metadata Fields
Sex, Time
View Samples- Rattus norvegicus
- 118 Downloadable Samples
- Affymetrix Rat Expression 230A Array (rae230a), Affymetrix Rat Genome U34 Array (rgu34a)
Description
The purpose of this study was to determine 1) the transcriptional program elicited by exposure to three estrogen receptor (ER) agonists: 17 a-ethynyl estradiol (EE), genistein (Ges) and bisphenol A (BPA) during fetal development of the rat testis and epididymis; and 2) whether very low dosages of estrogens (evaluated over five orders of magnitude of dosage) produce unexpected changes in gene expression (i.e., a non-monotonic dose-response curve). In three independently conducted experiments, Sprague-Dawley rats were dosed (s.c.) with 0.001-10mg EE/kg/day, 0.001-100 mg Ges/kg/day or 0.002-400mg BPA/kg/day. While morphological changes in the developing reproductive system were not observed, the gene expression profile of target tissues were modified in a dose-responsive manner. Independent dose-response analyses of the three studies identified 56 genes that are significantly modified by EE, 28 genes by Ges and 15 genes by BPA (out of 8740). Even more genes were observed to be significantly changed when only the high dose is compared with all lower doses: 141, 46 and 67 genes, respectively. Global analyses aimed at detecting genes consistently modified by all of the chemicals identified 52 genes whose expression changed in the same direction across the three chemicals. The dose-response curve for gene expression changes was monotonic for each chemical, with both the number of genes significantly changed and the magnitude of change, for each gene, decreasing with decreasing dose. Using the available annotation of the gene expression changes induced by ER-agonist, our data suggest that a variety of cellular pathways are affected by estrogen exposure. These results indicate that gene expression data are diagnostic of mode of action and, if they are evaluated in the context of traditional toxicological end-points, can be used to elucidate dose-response characteristics.
Publication Title
Gene expression changes induced in the testis by transplacental exposure to high and low doses of 17{alpha}-ethynyl estradiol, genistein, or bisphenol A.
Sample Metadata Fields
Sex, Age, Specimen part, Compound
View Samples