In this study we analyzed the effect of overexpression of an HA-tagged version of the ERF RAP2.12 on the transcriptome levels in aerobic and hypoxic-treated (O2 21% and 1%, respectively) Arabidopsis thaliana rosettes.
Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization.
Treatment
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Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway.
Age, Specimen part, Treatment
View SamplesThe effect of the overexpression of Plant Cysteine Oxidase (PDCO1) on the transcriptome of Arabidopsis resettes was investigated with plants subjected to a 4h hypoxia (5% O2 v/v in air). For this purpose, 4-week old rosette of wild-type and 35S:FLAG:CDO1 plants were compared. Samples were composed of pools of 5 plants.
Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway.
Age, Specimen part
View SamplesThe effect of the overexpression of Plant Cysteine Oxidase (PCO1) on the transcriptome of Arabidopsis resettes was investigated. For this purpose, 4-week old rosette of wild-type and 35S:FLAG:CDO1 plants were compared. Samples were composed of a pool of 5 plants.
Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway.
Age, Specimen part, Treatment
View SamplesThe effect of the overexpression of a stabilized version of the transcription factor RELATED TO APETALA2.12 (RAP2.12) on the transcriptome of Arabidopsis rosettes was investigated. To this purpose, 4-week old rosette of wild-type and 35S:13RAP2.12 plants were compared. Samples were composed of pools of 3 plants.
Age-dependent regulation of ERF-VII transcription factor activity in Arabidopsis thaliana.
Age, Specimen part
View SamplesGene expression was compared between 7-day old seedlings expressing two effector modules of an oxygen sensor device,consisting of the Saccharomyces cerevisiae Gal4 activation domain (AD) fused to the human HIF-CODD and the Gal4 DNA binding domain (BD) fused to the human pVHL, and seedlings expressing the previously described effector modules in addition to the sensory module constituted by the human PHD3 gene under control of constitutive promoters
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View SamplesHRE1 and HRE2 are two ERF transcription factors induced by low oxygen. In this work we analyzed the effect of ectopic expression of HRE1 and HRE2 on the arabidopsis transcriptome in aerobic and hypoxic (1% O2) conditions. While HRE1 has a moderate effect on the expression of anaerobic genes under hypoxia, HRE2 does not affect them either under aerobic or hypoxic conditions.
HRE1 and HRE2, two hypoxia-inducible ethylene response factors, affect anaerobic responses in Arabidopsis thaliana.
Age, Treatment
View SamplesAnoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia, heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis.
The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis.
Age, Treatment
View SamplesArabidopsis thaliana ecotype Columbia glabra were grown for 4 days in the dark without added sucrose. Samples were subsequently kept for 6h either [1] under aerobic conditions, [2] under anoxia in absence of sucrose or [3] under anoxia in presence of sucrose.
A genome-wide analysis of the effects of sucrose on gene expression in Arabidopsis seedlings under anoxia.
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View SamplesWe treated Arabidopsis seedlings with chitosan and carried out a transcript profiling analysis (GeneChip microarrays) in order to identify genes and transcription factors regulated by chitosan. The results showed that jasmonate and defense responsive genes, camalexin and lignin biosynthetic genes were among genes up-regulated by chitosan. Several transcription factors are also strongly induced by chitosan.
Transcript profiling of chitosan-treated Arabidopsis seedlings.
Age, Treatment
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