Filters
Technology
Platform
E-MEXP-739
Arabidopsis thaliana
24 Downloadable Samples
Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Powdery mildew, caused by the fungus Blumeria graminis (DC) Speer, is one of the most important foliar diseases of cereals worldwide. It is an obligate biotrophic parasite, colonising leaf epidermal cells to obtain nutrients from the plant cells without killing them. Syringolin A (sylA), a circular peptide secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers a hypersensitive cell death reaction (HR) at infection sites when sprayed onto powdery mildew infected wheat which essentially eradicates the fungus. The rational was to identify genes whose expression was specifically regulated during HR, i.e. genes that might be involved in the switch of compatibility to incompatibility.<br></br>Powdery mildew-infected or uninfected plants were treated with syringolin two days after infection and plant material for RNA extraction was collected at 0.5, 1, 2, 4, 8, 12 hours after treatment (hat), resulting in an early (2 and 4 hat) and late pool (8 and 12 hat). Plant material that was uninfected prior to syringolin treatment was collected 8 and 12 hat (late pool of uninfected plant material), and 1 hat, respectively.
Publication Title
Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites.
Sample Metadata Fields
Compound, Time
View Samples- Glycine max
- 9 Downloadable Samples
Illumina HiSeq 2000
Description
To provide novel insights into the molecular basis of floral initiation, RNASeq was used to characterize the soybean transcriptome of leaf and micro-dissected shoot apical meristem at different time points after short-day treatment.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Zea mays
- 4 Downloadable Samples
- Illumina HiSeq 2500, NextSeq 500
Description
RNA-Seq analysis of the drought responsive transcriptome of Zea mays cultivar Z59
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Arabidopsis thaliana
- 307 Downloadable Samples
- Illumina HiSeq 2000
Description
Time-course analysis of shade responsive genes in Col and 12 mutants.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Arabidopsis thaliana
- 182 Downloadable Samples
- Illumina HiSeq 2000
Description
Time-course data of shade avoidance in NAM parents
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Zea mays
- 38 Downloadable Samples
- Illumina HiSeq 2500
Description
Developmental gradient of expanding maize leaf
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part
View Samples- Zea mays
- 7 Downloadable Samples
- Illumina HiSeq 3000
Description
In order to study the gene expression of pollen tubes as they grow in silk after pollination, we pollinated maize W22 silks with maize B73 pollen. The recent (2016) advent of the W22 genome assembly and annotation allows us to single out RNA-seq reads originating from the pollen tubes. B73 pollen, W22 silk and B73 seedling controls were sequenced as well.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Arabidopsis thaliana
- 28 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Effects of loss-of-function of AtMIKC* MADS-box genes on the mature Arabidopsis pollen transcriptome.
Publication Title
MADS-complexes regulate transcriptome dynamics during pollen maturation.
Sample Metadata Fields
Age, Specimen part
View Samples- Arabidopsis thaliana
- 18 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Umkirch-1/Umkirch-3 hybrid plants and their parents were grown at 23SD and then shifted to 16SD for five days. 10 plants were pooled in each of three sample replicates.
Publication Title
Autoimmune response as a mechanism for a Dobzhansky-Muller-type incompatibility syndrome in plants.
Sample Metadata Fields
Specimen part
View Samples- Pseudomonas aeruginosa
- 18 Downloadable Samples
- Illumina HiSeq 2500
Description
RNASeq time course (1,2,3 hrs) of Pseudomonas aeruginosa (UCBPP-PA14) on plastic (polystyrene) petri dish
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Disease, Cell line
View Samples