To determine the role of RPX on cell proliferation and organ development, we performed microarray experiments in search of RPX target genes by using an estradiol-inducible RPXC protein.
An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana.
Specimen part
View SamplesTo identify genes that are regulated by SERF1, we performed expression profiling on roots of serf1 and wild-type plants under standard growth conditions.
SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice.
Specimen part
View SamplesTo understand the effect of high and low GA levels on plant metabolism and development in Arabidopsis we made use of the GA biosynthesis inhibitor paclobutrazol (PBZ) and exogenously applied GA. The whole genome response at the translation level was assessed by immunopurification of polysomes from PBZ- and GA treated plants expressing FLAG-tagged ribosomal protein L18 (RPL18B). Polysomal associated RNA was isolated and subjected to affymterix ATH1 CHIP analysis. A total of 140 genes were statistically determined to be differentially translated after GA treatment whereas 89 genes where affected PBZ treatment. Our analysis revealed that GA and PBZ have opposing effects on the expression of cell wall and wax layer biosynthesis related genes. In addition, many genes involved in secondary metabolism are upregulated upon PBZ treatment. A set of SAUR-like genes important for mediating auxin responses are downregulated by PBZ, which is of interest to coordinatian of GA levels with growth and development. Interestingly, GA treatment induces the upregulation of transcription factors related to plant defense and senescence, which is in agreement with the early flowering upon GA treatment. Our study provides a first picture of the response of Arabidopsis to altered GA levels at the translation level, and thus will be valuable for understanding gene regulation at the polysome level.
Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis.
Specimen part, Treatment
View SamplesTo identify potenital target genes of the transcription factor KUODA1 (AT5G47390), we performed expression profiling using an estradiol inducible overexpression system.
No associated publication
Age
View SamplesIdentification of ANAC059 and ANAC092 early target genes using inducible overexpression system coupled with investigation of T-DNA insertion mutants
No associated publication
No sample metadata fields
View SamplesThis work purposed on screening candidates of key genes invovled in the production of phenylacylated flavonol-glycosides
Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae.
No sample metadata fields
View SamplesGlobal transcriptome patterns were performed using ORE1-IOE-2h (2h after Estradiol and Mock treatment) as well as transiently (6h) overexpressed Arabidopsis mesophyll cell protoplasts
NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis.
Specimen part, Treatment
View SamplesGlobal transcriptome patterns were obtained from ATAF1-IOE seedlings at 1 h, 2 h and 5 h after estradiol induction or mock treatment, and from mature ATAF1-IOE leaves at 5 h after estradiol induction or mock treatment.
Transcription Factor Arabidopsis Activating Factor1 Integrates Carbon Starvation Responses with Trehalose Metabolism.
Specimen part, Treatment, Time
View SamplesTo identify genes associated with the heat stress (HS) memory, transcript profiling using Affymetrix ATH1 microarrays was performed to compare Col-0 seedlings after the priming stimulus with control plants (unprimed).
The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis.
Specimen part
View SamplesOs02g31890 encodes a dehydration-responsive transcription factor (named ARID) from rice (Oryza sativa, cv. Dongjin).
No associated publication
Specimen part
View Samples