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GSE155769
Pseudomonas aeruginosa
2 Downloadable Samples
Affymetrix Pseudomonas aeruginosa Array (paeg1a)
Description
In order to determine the effect of ALA on geneexpression, P. aeruginosa PAO1 was incubated with and without 30 μM ALA under T3SS-inducing conditions (calcium depletion by addition of NTA to growth medium), followed by RNA extraction and microarray analysis.
Publication Title
No associated publication
Sample Metadata Fields
Treatment
View Samples- Gallus gallus
- 17 Downloadable Samples
- Affymetrix Chicken Genome Array (chicken)
Description
Heat stress is a very complex physiological process. At present, there are some reports about physiological function and pathological changed on chicken heat stress.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Sus scrofa
- 9 Downloadable Samples
- Affymetrix Porcine Genome Array (porcine)
Description
Sow milk yield and quality is crucial for the survival and growth of piglets. Mammary epithelial cell proliferation, differentiation, milk component production and galactopoietic hormone and cytokine synthesis result from the quantities of differentially expressed genes.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Oryza sativa
- 4 Downloadable Samples
- Affymetrix Rice Genome Array (rice)
Description
To understand the molecular mechanism of drought stress resistance mediated by OsABA8ox3 gene, we checked the genome-wide expression profile changes in the OsABA8ox3 RNAi and WT seedlings using the Affymetrix GeneChip under the normal condition and drought stress. A total of 1436 genes showed greater than 2-fold higher expression levels in both WT and RNAi-9 seedlings after drought stress, and most of them had higher up-regulated folds in RNAi-9 seedlings than that of WT.
Publication Title
A key ABA catabolic gene, OsABA8ox3, is involved in drought stress resistance in rice.
Sample Metadata Fields
Specimen part, Time
View Samples- Oryza sativa indica group
- 3 Downloadable Samples
- Affymetrix Rice Genome Array (rice)
Description
Male sterility is an important trait in hybrid crop breeding. Thermo-sensitive genic male sterility (TGMS) lines, which are male-sterile at restrictive (high) temperatures but convert to male-fertile at permissive (low) temperatures, have been widely utilized in two-line hybrid rice breeding. However, the molecular mechanism underlying TGMS remains unclear. Here we show that the rice (Oryza sativa L.) thermo-sensitive genic male sterile gene 5 (tms5) locus, which in 2010 was present in cultivars occupying more than 71% (2.3 million hectares) of two-line hybrid rice-growing land in China, confers the TGMS trait through a loss-of-function mutation of RNase ZS1, resulting in failure to mediate mRNA decay of three temperature-responsive ubiquitin fusion ribosomal protein L40 genes (UbL40) genes. RNase ZS1, a member of the evolutionarily conserved endonuclease, processed tRNAs in vitro, but does not do so in vivo due to its localization in the cytoplasm. Defective RNase ZS1 in tms5 plants leads to over-accumulation of UbL401, UbL402 and UbL404 mRNAs at restrictive but not permissive temperatures. Over-expression of UbL401 and UbL404 in wild-type plants caused male sterility, whereas knockdown of UbL401 and UbL404 in tms5 plants partially restored the male fertility at restrictive temperatures. Our results uncover a novel mechanism of RNase ZS1-mediated UbL40 mRNA decay which controls TGMS in rice and has potential applications not only of rice but also of other crops.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Zea mays
- 14 Downloadable Samples
- Affymetrix Maize Genome Array (maize)
Description
Plant growth regulators (PGRs) were commonly used in practical farming to restrict plant height and control lodging. Ethephon has been reported to shorten the internodes elongation and increase the harvestable grain yield of maize. In the present study, we characterized that internodes phenotypic responses to ethephon treatment induced should due to ethylene released inhibited the longitudinal growth of cells and promoted its the lateral growth.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Arabidopsis thaliana
- 12 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Transcription profiling by array of Arabidopsis MKK9DD (constitutively active MKK9 kinase mutant) overexpressing seedlings and Pi-starved wild type seedlings to identify the same regulated genes
Publication Title
Activation of MKK9-MPK3/MPK6 enhances phosphate acquisition in Arabidopsis thaliana.
Sample Metadata Fields
Specimen part, Compound
View Samples- Gallus gallus
- 12 Downloadable Samples
- Affymetrix Chicken Genome Array (chicken)
Description
Adaptation to hypoxia is a complicated and important physiological course for organisms, but the genetic mechanism underlying the adaptation is not fully understood yet. Tibetan Chicken (T), an indigenous chicken breed in China which inhabit in high areas with an altitude above 2,900 meters. Shouguang Chicken(S) and Dwarf Recessive White Chicken (DRW), two lowland chicken breeds, were used as control groups. The heart was the first functional organ to develop during the embryonic development. Furthermore, the heart is an efficient energy converter utilizing the most appropriate fuel for a given environment. Therefore, GeneChip Chicken Genome Array was employed to identify the differentially expressed genes in embryonic hearts of Tibetan Chicken and two lowland chicken breeds in both hypoxic and normoxic incubating environments with a genome wide profile.
Publication Title
Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue.
Sample Metadata Fields
No sample metadata fields
View Samples- Arabidopsis thaliana
- 6 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
HUB2 had been previously identified as a H2B monoubiquitinating E3 ligase. Loss of HUB2 leads to a pleiotropic phenotype, including reduced seed dormancy, smaller plant size and early flowering. Microarray expression profiling was used to identify differentially expressed genes between hub2-3 mutant and wild-type Col seedlings.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Arabidopsis thaliana
- 6 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step towards a detailed understanding of this important process, the objective of this study was to comprehensively analyze the transcriptome changes during pollen germination and pollen tube growth. Using Affymetrix Arabidopsis ATH1 Genome Arrays, this study is the first to show the changes in the transcriptome from desiccated mature pollen grains to hydrated pollen grains and then to pollen tubes of Arabidopsis thaliana. The number of expressed genes, either for total expressed genes or for specifically expressed genes, increased significantly from desiccated mature pollen to hydrated pollen and again to growing pollen tubes, which is consistent with the finding that pollen germination and tube growth was significantly inhibited in vitro by a transcriptional inhibitor. The results of GO analyses showed that expression of genes related to cell rescue, transcription, signal transduction and cellular transport were significantly changed, especially for up-regulation, during pollen germination and tube growth, respectively. In particular, genes of the CaM/CML, CHX and Hsp families showed the most significant changes during pollen germination and tube growth. These results demonstrate that the overall transcription of genes, both in the number of expressed genes and in the levels of transcription, was increased. Furthermore, the appearance of many novel transcripts during pollen germination as well as tube growth indicates that these newly expressed genes may function in this complex process.
Publication Title
Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
Sample Metadata Fields
Age, Specimen part
View Samples