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GSE70619
Mus musculus
15 Downloadable Samples
Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
Hypercholesterolemai is a major contributor to atherosclerosis development. To assess the effects of hypercholesterolemia on the transcriptional profiling in foam cells, mice were fed regular chow, or WD for 2 or 14 weeks prior to sacrifice.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 6 Downloadable Samples
- Affymetrix Mouse Gene 2.0 ST Array (mogene20st)
Description
Group-2 innate lymphoid cells (ILC2) are tissue-resident, long-lived innate effector cells implicated in allergy and asthma. Upon activation, mature ILC2 rapidly secret large amounts of type-2 cytokines and other effector molecules. The molecular pathways that drive ILC2 activation are not well understood. Here we report that the transcriptional controller Core-binding factor (CBF) is required for ILC2 activation. Deletion or inhibition of CBF did not impair the maintenance of ILC2 at homeostasis, but abolished ILC2 activation during allergic airway inflammation. Treatment with CBF inhibitors prevented ILC2-mediated airway hyperresponsiveness in a mouse model of acute Alternaria allergen inhalation. CBF promoted expression of key ILC2 genes at both transcriptional and translational levels. CBF transcriptional complex directly bound to Il13 and Vegfa promoters and enhancers, and controlled gene transcription. CBF further promoted ribosome biogenesis and enhanced gene translation in activated ILC2. Together, these data establish an essential role for CBF in ILC2 activation.
Publication Title
No associated publication
Sample Metadata Fields
Cell line
View Samples- Arabidopsis thaliana
- 12 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
We carried out microarray analysis to investigate the global gene expression pattern in response to supplemental far-red light under different temperature contexts.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Arabidopsis thaliana
- 12 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Microarray analysis to investigate the global gene expression patterns in response to ABA under different light quality backgrounds
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 6 Downloadable Samples
- Affymetrix Mouse Gene 2.0 ST Array (mogene20st)
Description
Understanding the molecular mechanisms defining and maintaining the identity of a specific neuronal cell type is a central goal in neuroscience. The vomeronasal organ (VNO) of mice contains hundreds of distinct vomeronasal sensory neurons (VSNs). The VSNs are classified into two major cell types that are segregated in apical and basal regions of the VNO, express vomeronasal receptors of different superfamilies, and send axons to different portions of the accessory olfactory bulb. How apical or basal identity of VSNs is established and maintained is largely unknown. Here we attempt to assess the role of a single transcription factor, AP-2, in the VNO of mice.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Homo sapiens
- 6 Downloadable Samples
Illumina HiSeq 2500
Description
This work illustrates that clusters of circRNAs are aberrantly expressed in HBV-related hepatocellular carcinoma (HCC), which might provide potential targets for the early diagnosis of this disease and new genetic insights into HCC.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Danio rerio
- 2 Downloadable Samples
- IlluminaHiSeq4000
Description
Patients taking glucocorticoid or glucocorticoid-like drugs for an extended period of time can develop osteoporosis, termed glucocorticoid-induced osteoporosis (GIOP). GIOP is the most common form of secondary osteoporosis, but the mechanism underlying its development is unclear. In the present study, we used prednisolone to treat zebrafish larvae to investigate GIOP. Our RNA deep-sequencing (RNA-seq) results show that prednisolone affects genes known to act in the extracellular region, and therefore the extracellular region, extracellular matrix, and collagen trimer might be involved in glucocorticoid-induced osteoporosis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the focal adhesion signaling pathway is the most enriched signaling pathway in terms of differentially expressed genes (DEGs). In this pathway, two adapter proteins, itga10 and itgbl1, were down-regulated in the prednisolone-treated larvae. Further experiments showed that these two genes contribute to glucocorticoid-induced osteoporosis. The results of our study provide new insights into glucocorticoid-induced osteoporosis.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 154 Downloadable Samples
- Affymetrix Human Exon 1.0 ST Array [GENCODE v10 (huex10st)
Description
These samples are being analyzed by the Duke-UNC-Texas-EBI ENCODE consortium. Expression from these cell types will compared to three whole genome open chromatin methodologies: DNaseI hypersensitivity (DNase-seq), Formaldehyde-Assisted Isolation of Regulatory elements (FAIRE-seq), and Chromatin Immunoprecipitation (ChIP-seq) .
Publication Title
Heritable individual-specific and allele-specific chromatin signatures in humans.
Sample Metadata Fields
Specimen part
View Samples- Homo sapiens
- 18 Downloadable Samples
- Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
No associated publication
Sample Metadata Fields
Cell line
View Samples- Homo sapiens
- 39 Downloadable Samples
- Affymetrix Human Exon 1.0 ST Array [transcript (gene) version (huex10st)
Description
These samples are part of the ENCODE consortiums proposed time-limited Pilot Study for confirmation of the utility of RNA abundance measurements as a standard reference phenotyping tool.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples