publication_authors:Vreugdenhil E Results

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Microarray (23)

Platform

Affymetrix Mouse Gene 2.0 ST Array (mogene20st) (26)

Affymetrix Mouse Gene 1.0 ST Array (mogene10st) (20)

Affymetrix Mouse Genome 430 2.0 Array (mouse4302) (10)

Affymetrix Human Gene 2.0 ST Array (hugene20st) (3)

Includes Publication

GSE8349

Microarray platform comparison study of hippocampal gene expression in DCLK1 transgenic and wild-type mice

Mus musculus
10 Downloadable Samples
Affymetrix Mouse Genome 430 2.0 Array (mouse4302)

Description

The aim of the present study was to compare, on a statistical basis, the performance of different microarray platforms to detect differences in gene expression in a realistic and challenging biological setting. Gene expression profiles in the hippocampus of five wild-type and five transgenic C-doublecortin-like kinase mice were evaluated with five microarray platforms: Applied Biosystems, Affymetrix, Agilent, Illumina and home-spotted oligonucleotide arrays. We observed considerable overlap between the different platforms, the overlap being better detectable with significance level-based ranking than with a p-value based cut-off. Confirming the qualitative agreement between platforms, Pathway analysis consistently demonstrated aberrances in GABA-ergic signalling in the transgenic mice, even though pathways were represented by only partially overlapping genes on the different platforms.

Publication Title

Can subtle changes in gene expression be consistently detected with different microarray platforms?

Sample Metadata Fields

No sample metadata fields

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GSE62291

Cellular retinoic acid binding protein 2 (CRABP2) inhibits tumor growth by two distinct mechanisms

Homo sapiens
3 Downloadable Samples
Affymetrix Human Gene 2.0 ST Array (hugene20st)

Description

CRABP2 potently suppresses carcinoma cell growth, yet the mechanism(s) that underlie this activity remain incompletely understood. Two distinct functions are known for CRABP2: 1) the classical function of this protein is to directly deliver retinoic acid (RA) to the nuclear retinoic-acid receptorthereby activate gene expression, and 2) in the absence of RA, CRABP2 directly binds to the RNA-binding and stabilizing protein, HuR, and markedly strengthens its interactions with target mRNAs.

Publication Title

Cellular retinoic acid-binding protein 2 inhibits tumor growth by two distinct mechanisms.

Sample Metadata Fields

Specimen part, Cell line

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GSE93371

Transcriptomic comparison of FVB mouse strain lung Cells one week upon injecting mice intraperitoneally with urethane and with the mouse lung adenocarcinoma cell line FULA 1

Mus musculus
3 Downloadable Samples
Affymetrix Mouse Gene 2.0 ST Array (mogene20st)

Description

Transcriptomic comparison of FVB mouse strain lung Cells one week upon injecting mice intraperitoneally with either saline or Urethane. Mouse lung cell were also compared at the transcriptomic level with the mouse lung adenocarcinoma cell line FULA 1, which was established in our lab