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SRP116930
Homo sapiens
22 Downloadable Samples
Illumina HiSeq 2000
Description
In this project we analyze the transcriptome of the human multiple myeloma isogenic cell lines ARP-1 (UTX wild-type) and ARD (UTX null). The transcriptome is studied at baseline, upon restoration of UTX levels in ARD cells for 3 and 6 days, and upon treatment of the cell lines with the EZH2 inhibitor GSK343. Moreover, we analyzed the transcriptome of a ARD resistant cell line that we generated. Overall design: Examination of transcriptome of two cell lines upon restoration of UTX and treatment with EZH2 inhibitors
Publication Title
UTX/KDM6A Loss Enhances the Malignant Phenotype of Multiple Myeloma and Sensitizes Cells to EZH2 inhibition.
Sample Metadata Fields
Specimen part, Subject
View Samples- Drosophila melanogaster
- 36 Downloadable Samples
Affymetrix Drosophila Genome 2.0 Array (drosophila2)
Description
Dentatorubral-pallidoluysian Atrophy (DRPLA) is a human polyQ disease caused by the expansion of a CAG strech in the atrophin-1 (at-1) gene. In all vertebrates, a second atrophin gene (at-2) is present and it encodes a related protein void of polyQ tracks. In D.melanogaster there is one conserved Atrophin (Atro) gene, ubiquitously expressed, which contains all functional domains of vertebrate Atrophins, including two polyQ stretches. To understand to what extent transcriptional alterations cause neurodegeneration and are linked to the normal functions of Atrophin, we performed a genome wide transcriptional profiling in our Drosophila models, focusing on primary events that precede neurodegeneration.
Publication Title
Polyglutamine Atrophin provokes neurodegeneration in Drosophila by repressing fat.
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