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Accession IconSRP198408

RNA sequencing in human GBM stem cells with Myc knockdown and PARP inhibitor treatment

Organism Icon Homo sapiens
Sample Icon 16 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
This experiment is to examine the effect of PARP inhibitor and Myc shRNA knockdown on transcriptome profiles in MYC-amplified human GBM stem cells MGG4. Overall design: There are totally 4 samples. GBM cell MGG4 expressing scramble shRNA or shRNA targeting Myc were grown in doxycycline (Dox, 1 mg/ml) for 6 days, treated with olaparib (Ola, 10 microM) or DMSO for 24h, and harvested for RNA extraction, followed by RNA sequencing
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16
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