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Accession IconSRP162335

Microtubule acetylation is required for mechanosensation in Drosophila

Organism Icon Drosophila melanogaster
Sample Icon 28 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
At the cellular level, a-tubulin acetylation alters the structure of microtubules to render them mechanically resistant to compressive forces. How this biochemical property of microtubule acetylation relates to mechanosensation remains unknown, though prior studies have shown that microtubule acetylation influences touch perception. Here, we identify the major Drosophila a-tubulin acetylase (dTAT) and show that it plays key roles in several forms of mechanosensation. dTAT is highly expressed in the larval peripheral nervous system (PNS), but is largely dispensable for neuronal morphogenesis. Mutation of the acetylase gene or the K40 acetylation site in a-tubulin impairs mechanical sensitivity in sensory neurons and behavioral responses to gentle touch, harsh touch, gravity, and vibration stimuli, but not noxious thermal stimulus. Finally, we show that dTAT is required for mechanically-induced activation of NOMPC, a microtubule-associated transient receptor potential channel, and functions to maintain integrity of the microtubule cytoskeleton in response to mechanical stimulation. Overall design: Six neuronal and non-neuronal cell types of Drosophila melanogaster larvae, with 100 cells each and at least four biological replicates were profiled by mRNA-Seq
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28
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