Total RNA was extracted from the endometrial and placental samples in duplicate using a RNeasy Plus Universal Mini Kit (Qiagen, Valencia, CA) and all extractions were performed according to the manufacturers instructions. An aliquot of each sample was qualitatively assayed for purity using UV spectroscopy via the Nanodrop8000 (ThermoFisher Scientific, Waltham, MA) to determine the 260/280 and 260/230 ratios, respectively. RNA integrity was measured using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). All RNA purity ratios, were > 1.5 and all RNA integrity numbers (RIN) were approximately 6. Total RNA was quantitated with the Broad Range Assay in the Qubit (ThermoFisher Scientific, Waltham, MA). Each sample was normalized to a standard input concentration of 2 _g for sequencing library preparation. Stranded mRNA sequencing libraries were prepared manually with the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) following the manufacturers recommended procedures.

Illumina HiSeq 2500 paired end sequencing; RNAseq of Sus scrofa: low efficiency placenta

15-R3

To elucidate potential mechanisms, morphological measurements and gene expression profiles in placental and associated endometrial tissues of high PE and low PE feto-placental units were compared.

Sus scrofa breed:Camborough 23 x PIC 1025 Raw sequence reads

Submitter Supplied Information

Samples