Single-cell RNA-seq of NR5A1-eGFP positive cells of the developing mouse ovary from E10.5 to P6

Purpose: We performed a time-course single-cell RNA-seq of the somatic cells of the XX mouse gonads to study the cell population heterogeneity and the genetic program during their differentiation. Methods: We collected gonads from NR5A1-eGFP transgenic embryos at six embryonic stages: E10.5, E11.5, E12.5, E13.5, E16.5 and P6. Methods: Cells were capture with the C1 autoprep system and cDNA sequenced with Illumina HiSeq 2000. Results: One cell population was detected at E10.5 and give rise to both Granulosa and steroidogenic precursor cells. A precursor cell population remains undifferentiated at P6 and are likely to be theca cell precursors. Conclusion: Our study is, to date, the most granular transcriptomic study of the developing mouse ovary and provide a more complete model of somatic cell differentiation during female sex determination. Overall design: 663 cells were collected in total. 71 cells at E10.5, 106 cells at E11.5, 164 cells at E12.5, 106 cells at E13.5, 95 cells at E16.5, and 121 at P6. We performed two independent captures for each embryonic stage to reach a reasonable number of cells except for E10.5 where we capture enough cells in one experiment.

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Stévant I, Kühne F, Greenfield A, Chaboissier MC, Dermitzakis ET, Nef S