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Accession IconSRP159650

Single-cell RNA-seq analysis of human tonsil CD14+ cells

Organism Icon Homo sapiens
Sample Icon 1 Downloadable Sample
Technology Badge IconIllumina HiSeq 2500

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Description
We performed single-cell RNA-seq on CD14+ cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology. Overall design: Tonsil phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells and macrophages were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer's protocol.
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