Influence of miR-21-regulated pathways on T cell responses

The goal of this study was to examine how miR-21 upregulation influences T cell responses. miR-21 was antagonized by lentiviral overexpression of anti-sense miR-21 during activation of naive CD4 T cells from healthy adults. On day 5 after activation, libraries of miR-21 high and miR-21 low cells were prepared using the Ovation Human FFPE RNA-Seq Library Systems (NuGEN) and sequenced on an Illumina HiSeq 2500.

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