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Accession IconSRP158196

AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein

Organism Icon Homo sapiens
Sample Icon 8 Downloadable Samples
Technology Badge IconIon Torrent S5

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Description
We compared polyIC stimulated cells in the presence of either GFP or NS5 protein Overall design: A549 cells presence with GFP- or NS5-expressing plasmid using Polyjet (Signagen) according to manufacturer instructions, and 30 hours later stimulated with polyIC (Tocris) as previously described (Marazzi et al., 2012). At 12 hours post-stimulation, total cellular RNA was purified by RNeasy column (Qiagen).
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8
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