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Accession IconSRP151477

Deciphering the C. elegans embryonic transcriptome with tissue, time, and alternative splicing resolution

Organism Icon Caenorhabditis elegans
Sample Icon 82 Downloadable Samples
Technology Badge IconNextSeq 500

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We have used FACS to isolate fluorescent cells at multiple time points from synchronized embryos containing early and highly specific tissue/lineage markers. We then carried out RNA-seq, and observe dramatic differences in gene expression levels both between cell-types, and over time within the same population. Furthermore, we observe differential transcript usage between cell-types and over time, including differential promoter and differential exon usage that leads to additional differences between cell types.
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