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Accession IconSRP149366

Estrogen responsive transcriptome of estrogen receptor positive normal human breast cells in 3D cultures

Organism Icon Homo sapiens
Sample Icon 38 Downloadable Samples
Technology Badge IconIllumina HiSeq 3000

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Description
Understanding how differentiation, microenvironment, and hormonal milieu influence human breast cell susceptibility to malignant transformation will require the use of physiologically relevant in vitro systems. We developed a 3D culture model that enables the propagation of normal estrogen receptor alpha (ER)+ cells. The purpose of this experiment was to assess ER functionality and compare estrogen-induced transcripts among samples and systems. Overall design: RNA-seq was performed on RNA prepared from replicate 3D cultures from 3 normal 3D breast culture specimens exposed to 10nM estradiol or vehicle alone for 6 or 24 hours.
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39
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