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Accession IconSRP147931

Determination of global decay rates of yeast transcriptome and identification of factors impact mRNA stability

Organism Icon Saccharomyces cerevisiae
Sample Icon 10 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
In this work, we determine total mRNA decay rates in rpb1-1 and rpb1-1/caf1? cells, calculate half-lives in rpb1-1/caf1? cells relative to rpb1-1 cells and correlate them with codon optimality. Overall design: mRNA profiling was done on 10 time points in rpb1-1/caf1 cells and sequenced using a paired end protocol on an Illumina HiSeq2000 instrument. A biological duplicate was performed.
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10
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