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Accession IconSRP144076

Nascent RNA Sequencing after NMYC activation in SH-EP MYCNER cells

Organism Icon Homo sapiens
Sample Icon 6 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
In order to distinguish transcription changes from RNA modification and post transcription changed, nascent RNA seq via metabolic labeling of freshly synthesized RNA was carried out using 4sU labeling/biotin purification. Overall design: nascent RNA was extractred post N-MYC activation and compared with untreated cells nascent RNA to gather fold changes of pre-mRNA on gene basis.
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6
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