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Accession IconSRP142614

RNA-seq of CD33 KO and control HSPCs

Organism Icon Homo sapiens
Sample Icon 10 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
Gene expression profile of in vitro differentiated control and CD33 KO CD34+ cells (with 70-85% CD33 KO) were analyzed by RNA-seq to exclude any major impact of CD33 loss on downstream gene expression Overall design: Primary CD34+ cells were treated with CRISPR/Cas9 to disrupt the CD33 gene and grown in culture for 5-7 days prior to analysis; mRNA profile was compared to control cells from the same donor that were also treated with Cas9 and a control gRNA; 5 different donors were evaluated (CD33 KO/control for each = total 10 samples)
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10
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No associated institution

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