Illumina HiSeq 2500 paired end sequencing; GSM3068996: SLX_13258.i710_i517; Sus scrofa; RNA-Seq

SLX_13258.i710_i517

we report single cell expression profiles of embryonic cells (from day 5 to 11) of pig embryo development. Overall design: single cell transcriptomes were generated from 220 cells obtained from 28 embryos (15 male and 13 female)

Regulation of lineage segregation, pluripotency and X chromosome inactivation in the pig revealed by scRNA-Seq

Submitted on 30-JAN-2019

Single cells were collected manually into lysis buffer following the SMARTSEQ. 2 protocol published by Picelli et al., 2014 (Nat. Protocols 9, 171-181) Libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina), as used Picelli et al., 2014..

Regulation of lineage segregation, pluripotency and X chromosome inactivation in the pig revealed by scRNA-Seq

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