github link
Accession IconSRP134974

Effect of transgenic RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

Organism Icon Drosophila melanogaster
Sample Icon 70 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer IIx

Submitter Supplied Information

Description
We compared four transcription factor knockdowns using transgenic RNAi expressed in the larval fat body. FOXO, Tfb1, p53, and Stat92E-dependent gene expression in the Drosophila fat body was quantified on control and high-sugar diets in order to generate expression profiles via RNA-seq. These expression data were used to build a gene regulatory network to predict novel roles for these and other genes during caloric overload. Overall design: Control and fat body-expressed transcription factor RNAi Drosophila were reared on control (0.15M sucrose) and high-sugar (0.7M or 1M sucrose) diets until the wandering stage. Fat bodies were isolated and RNA extracted to determine the effects of diet on gene expression using Illumina RNA-seq.
PubMed ID
Total Samples
71
Submitter’s Institution
No associated institution

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Sex
Specimen part
Treatment
Subject
Processing Information
Additional Metadata
No rows found
Loading...