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Accession IconSRP119979

Transcriptional characterisation of the nuclear reprogramming process of fibroblasts, neutrophils and keratinocytes into induced pluripotent stem cells.

Organism Icon Mus musculus
Sample Icon 42 Downloadable Samples
Technology Badge IconIllumina HiSeq 1500

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Description
Nuclear reprogramming is an inefficient process with only a small proportion of cells successful converting into induced pluripotent stem (iPS) cells. However, in order to molecularly understand the process these rare intermediates need to be identified and isolated for profiling. In the context of this project we purified the rare reprogramming for three cell types (Fibroblasts, Neutrophils and Keratinocytes) by fluorescent activated cell sorting and submitted them, together with the resulting iPS cells, to RNA sequencing.
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