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Accession IconSRP118933

Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils

Organism Icon Mus musculus
Sample Icon 7 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
RNAseq (3''DGE) profiles of osteoblasts from four lung cancer-bearing mice and three tumor-free mice. Overall design: Osteoblasts were FACS-sorted using the following markers: CD45-CD31-Terr119-GFP+ from lineage depleted bone and bone marrow tissue of lung tumor-bearing or tumor-free age-, sex- and litter-matched KrasLSL-G12D/WT;p53Flox/Flox (KP)-Ocn GFP mice. Total RNA was prepared using the Trizol method followed cDNA preparation, amplification, Illumina adapter ligation and 3''end sequencing by Illumina HiSeq 2500
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7
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