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Accession IconSRP111509

RNA-Seq expression profiling of mouse DEN-initiated hepatocytes with or without Tgfbr2 inactivation

Organism Icon Mus musculus
Sample Icon 2 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Initiated hepatocytes (IHCs), isolated from DEN exposed Tgfbr2 flox/flox mouse, were infected with GFP or Cre-expressed adenovirus, followed by 1ng/ml TGFß × 48 hours incubation. Then total RNAs were isolated and processed to routine sequencing on Illumina platform. RNA-Seq libraries were prepared from total RNA using polyA enrichment. Overall design: Examination of transcriptome differences between initiated hepatocytes with or without Tgfbr2 inactivation.
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