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Accession IconSRP105771

Next Generation Sequencing measuring Nod-like receptors mRNA expression in intestinal epithelial cells

Organism Icon Mus musculus
Sample Icon 2 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Intestinal epithelisal cells were obtained by EDTA isolation of ileum from WT mice followed by facs sorting (EpCAM + CD45 – 7AAD – ). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat , and gene expression levels were measured by Cufflinks . Overall design: Ileum epithelisal cells mRNA profiles were generated by deep sequencing, in duplicate, using illumina HiSeq 2000.
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2
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