High throughput quantitative whole transcriptome analysis of individual macrophages 7 days post-sham thoracotomy in B6 CSF1R-GFP mice

Using fluorescence activated cell sorting, we isolated CD45+, CSF1R-GFP+, F4/80+, Ly6G- mouse lung monocytes and macrophages at 7 days after sham thoracotomy procedures. We then used microfluidic single cell RNA-sequencing to transcriptional profile unique myeloid subsets. Overall design: After sequencing 31 single cell transcriptomes were analyzed. Hierarcical and k-means clustering reveals several populations of macrophages are present in the lung.

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Lechner AJ, Driver IH, Lee J, Conroy CM, Nagle A, Locksley RM, Rock JR