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Accession IconSRP100788

Single cell RNA-seq of 444 epithelial cells from the mammary glands of pubescent and adult mice

Organism Icon Mus musculus
Sample Icon 422 Downloadable Samples
Technology Badge IconNextSeq 500

Submitter Supplied Information

Description
Mammary glands were collected from 8 pubescent (4.7-4.9 week-old) female mice and 8 adult (10 week old) female mice. Freshly sorted epithelial cells were submitted to a Fluidigm C1 System machine for single cell capture and cDNA synthesis. Cells were visualized under the microscope to ensure integrity of the captured single cells prior to cDNA preparation. Libraries were prepared using the Nextera XT kit and sequencing was carried out on an Illumina NextSeq 500 to achieve paired-end 75 bp reads. Overall design: RNA-seq profiling was completed for 221 cells from pubescent mice and 223 cells from adult mice.
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444
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No associated institution

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