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Accession IconSRP096989

Next Generation Sequencing of splenic- and CNS-infiltrating OT-1 cells in ODC-OVA mice upon LCMV-OVA and Lm-OVA infection

Organism Icon Mus musculus
Sample Icon 18 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goal of this study is to compare the transcriptome of OT-1 cells during priming (3 days after infection) and during effector phase ( 7 days after infection) in ODC-OVA mice after LCMV-OVA and Lm-OVA infection Overall design: OT-1 mRNA profiles of 7-weeks old ODC-OVA mice adoptively transfered with 10^5 OT-1 CD45.1 cells 3 and 7 days after i.v. infection with LCMV-OVA and Lm-OVA (deep sequencing, in triplicate, using Illumina).
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18
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