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Accession IconSRP093386

RNA-seq analysis of ESR1 mutations in T47D and MCF7 cell lines

Organism Icon Homo sapiens
Sample Icon 48 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Purpose: Transcriptome analysis of ESR1 mutant cells was performed via sequencing total RNA in T47D and MCF7 cell lines containing Y537S and D538G mutations. Overall design: Methods: Individual ESR1 WT and mutant T47D and MCF7 clones were hormone deprived in CSS for three days, pooled, and plated in quadruplicates in 6-well plates. The cells were treated with veh or 1nM E2 for 24 hrs, RNA was isolated and subjected to sequencing. Differential expression analysis was performed by DEseq2 and R was used to conduct statistical analysis tests
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48
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