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Accession IconSRP081265

Escape from X-Inactivation Tumor Suppressor (EXITS) genes contribute to sex bias

Organism Icon Mus musculus
Sample Icon 9 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
In this manuscript, we described male-biased mutations in chrX genes in cancer. In this RNA-seq experiment we tested the transcriptional consequences of shRNA knockdown of one of those genes, CNKSR2 Overall design: Murine NIH 3T3 cells were infected with and selected for expression of lentiviruses expressing shRNAs targeting Cnksr2 (2 independent shRNA sequences) or a control shRNA (targeting RFP, a gene not present in these cells). Each was performed in biological triplicate independent cultures for n=9 total samples
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9
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