Gene expression profiling of adipocyte precursor cells in response to Pdgfa

Adipocyte precursor cells were treated with Pdgfa during 1 or 2 hours in vitro to identify early changes in transciprion in response to treatment. This experiment supports the evidence that Pdgfa induces proliferation and maintenance of adipocyte stem cells. Overall design: Adipocyte precursor cells were isolated by FACS and treated with 30ng/ml of recombinant mouse Pdgfa for 1 or 2 hours.

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Rivera-Gonzalez GC, Shook BA, Andrae J, Holtrup B, Bollag K, Betsholtz C, Rodeheffer MS, Horsley V