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Accession IconSRP076721

RNA-Seq analysis of wild-type and Brpf1-null fetal liver LSK cells

Organism Icon Mus musculus
Sample Icon 8 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
To compare the impact of hematopoietic-specific Brpf1 gene inactivation, LSK (Lin-Sca1+cKit1+) cells were sorted from wild-type and Brpf1-null fetal liver cells for RNA-Seq. Overall design: Four E14.5 embryos were used to pool sufficient LSK cells for total RNA isolation and subsequent sequencing on HiSq2500. Two independent pairs of wild-type and mutant RNA samples (each of which contained LSK cells pooled from four embryos) were used for oligo-dT primed RNA Seq.
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8
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