Expression profile of TRAMP-C1 cell line with PAX8-NFE2L2 overexpression

We synthesized the PAX8-NFE2L2 fusion transcript and cloned it into a lentiviral vector, and used this to overexpress it in the murine prostate adenocarcinoma cell line TRAMP-C1. Overall design: We used high coverage RNA sequencing (>30 million reads per sample) to compare the expression profiles of cells expressing the PAX8-NFE2L2 fusion transcript to cells transduced with an empty vector.

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Alaei-Mahabadi B, Bhadury J, Karlsson JW, Nilsson JA, Larsson E