Allele specific deletion of enhancer clusters within mouse F1 embryonic stem cells

We provide data from several targeted deletions of transcriptional enhancer clusters within mouse F1 embryonic stem (ES) cells. We targeted these regions for deletion with CRISPR/Cas9 genome editing tools. We demonstrate through heterozygous enhancer cluster deletion and allele specific RNA-seq that enhancer clusters differ in their regulatory activity as the magnitude of the observed change in transcription upon enhancer cluster deletion varies greatly. Overall design: Strand specific RNA-seq after heterozygous or homozygous enhancer cluster deletion in mouse F1 ES cells (M. musculus129 x M. castaneus)

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Moorthy SD, Davidson S, Shchuka VM, Singh G, Malek-Gilani N, Langroudi L, Martchenko A, So V, Macpherson NN, Mitchell JA