github link
Accession IconSRP071898

Allele specific deletion of enhancer clusters within mouse F1 embryonic stem cells

Organism Icon Mus musculus
Sample Icon 32 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

Submitter Supplied Information

Description
We provide data from several targeted deletions of transcriptional enhancer clusters within mouse F1 embryonic stem (ES) cells. We targeted these regions for deletion with CRISPR/Cas9 genome editing tools. We demonstrate through heterozygous enhancer cluster deletion and allele specific RNA-seq that enhancer clusters differ in their regulatory activity as the magnitude of the observed change in transcription upon enhancer cluster deletion varies greatly. Overall design: Strand specific RNA-seq after heterozygous or homozygous enhancer cluster deletion in mouse F1 ES cells (M. musculus129 x M. castaneus)
PubMed ID
Total Samples
33
Submitter’s Institution
No associated institution

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Cell line
Subject
Processing Information
Additional Metadata
No rows found
Loading...