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Accession IconSRP068967

Ribosomal footprinting of CN34-Parental and CN34-LM1a

Organism Icon Homo sapiens
Sample Icon 8 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
We used Illlumina Artseq Ribo Profile to perform ribosome footprinting on the above cell lines Overall design: We followed the manufacturer's protocol: Cells were treated with cycloheximide (0.1 mg/ml) to inhibit translation; total RNA(tt) and ribosome-protected RNA fragments (RPF) were isolated and library preped for sequencing simultaneously.
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8
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