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Accession IconSRP068744

HDAC3 modulates enhancer activity to regulate terminal B cell differentiation

Organism Icon Mus musculus
Sample Icon 8 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
B220+GL7+ (GC) and B220+GL7- (non-GC) B cells were sorted from SRBC-immunized mice deficient for Hdac3 and wild type controls. RNA-sequencing revealed an upregulation of critical regulators of B cell differentiation in Hdac3-deleted animals. Overall design: 10 days post-immunization with SRBCs, GC and non-GC B cells were sorted and RNA isolated by Trizol extraction for RNA-sequencing. 2 replicates were sequenced for each condition.
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