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Accession IconSRP067154

Sequencing Universal Human Reference RNA by Smart-seq and early barcoding library preparation methods

Organism Icon Homo sapiens
Sample Icon 11 Downloadable Samples
Technology Badge IconIllumina HiSeq 1500

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Description
Many library preparation methods are available for gene expression quantification. Here, we sequenced and analysed Universal Human Reference RNA (UHRR) prepared using Smart-Seq2, TruSeq (public data) and a protocol using unique molecular identifiers (UMIs) that all include the ERCC spike-in mRNAs to investigate the effects of amplification bias on expression quantification. Overall design: UHRR 10 and 12 replicates for Smart-seq2 and UMI-seq library preparation methods, respectively.
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11
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No associated institution

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