Effect of PD-1 immune checkpoint in Alzheimer''s disease transgenic mice

We examined the brain''s choroid plexus and myeloid cell populations isolated from the brain of 5XFAD Alzheimer''s disease transgenic mice following PD-1 blockade Overall design: Choroid plexus samples and myeloid cell populations were isolated from the brain of 5XFAD mice following PD-1 blockade, and sequenced. For choroid plexus samples, 5 mice were treated with anti-PD-1, 5 with IgG control, and 4 were left untreated. For the myeloid cells samples, myeloid cells sorted from the brains of 5XFAD mice according to a gating strategy that seperate microglia (CD11b+CD45-low) and monocytes-derived macrophages (CD11b+CD45-high).

25

Baruch K, Deczkowska A, Rosenzweig N, Tsitsou-Kampeli A, Sharif AM, Matcovitch-Natan O, Kertser A, David E, Amit I, Schwartz M