NextSeq 500 sequencing; GSM1945143: T0.10000nM_Halofuginone_1; Mus musculus; RNA-Seq

T0.10000nM_Halofuginone_1

Bone marrow derived macrophages treated with small molecules and stimulated with LPS Overall design: Wild-type BMDMs pretreated with small molecules for 30 minutes prior to stimulation with LPS

Simultaneous pathway activity inference and global gene expression analysis using RNA-sequencing [halofuginone]

Submitted by Gene Expression Omnibus on 07-MAY-2016

RLT lysis, SPRI purification, Turbo DNase, MaximaRT, Random hexamer 2nd strand cDNA 50% of total first strand cDNA, 1ng of ds_cDNA per 96-well plate, 12 minutes and run for 16 cycles PCR according to Nextera manufactures protocol Modified SCRB-Seq, Soumillon, M., Cacchiarelli, D., Semrau, S., Oudenaarden, A. van & Mikkelsen, T. S. Characterization of directed differentiation by high-throughput single-cell RNA-Seq. bioRxiv 003236 (2014). doi:10.1101/003236; Modified Nextera protocol to PCR enrich only  the 3' end of mRNA transcripts and create digital gene expression libraries for Illumina sequencing.

Simultaneous pathway activity inference and global gene expression analysis using RNA-sequencing [halofuginone]

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