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Accession IconSRP065879

Effect of Cited2 knockdown on global transcript expression in Rcho-1 cell differentiation

Organism Icon Rattus norvegicus
Sample Icon 6 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We had previously discovered that the transcription factor Cited2 was highly induced during trophoblast differentiation. In this study, we used an lentiviral shRNA strategy to decrease Cited2 expression in Rcho-1 trophoblast cells. A RNA-seq approach was used to determine global transcript differences inRcho-1 knockdown cells compared to control cells. Overall design: Rcho-1 cells transduced with control shRNAs were used as controls. Cells transduced with shRNAs targetingCited2 were used as treatment.Cells were differentiated for 8 days and the analyses were done.
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6
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