Comparative analysis of Rtf1- and PAF1C-regulated genes

Paf1 and Ski8 were selected as representative subunits of the Paf1 complex (PAF1C), and RNA-seq analysis was performed in triplicate to compare the genes affected by Paf1, Ski8, and Rtf1 knockdown in HeLa cells. Overall design: Total RNA was harvested from control HeLa and Ski8 knockdown cells at day 4 and from Rtf1 or Paf1 knockdown cells at day 7 and was subjected to RNA-seq in triplicates.

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Cao QF, Yamamoto J, Isobe T, Tateno S, Murase Y, Chen Y, Handa H, Yamaguchi Y