github link
Accession IconSRP062936

Swedish mutation within amyloid precursor protein modulates global gene expression towards the pathogenesis of Alzheimer''s disease

Organism Icon Homo sapiens
Sample Icon 4 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer II

Submitter Supplied Information

Description
The Swedish mutation (K595N/M596L) of amyloid precursor protein (APP-sw) has been known to increase abnormal cleavage of cellular APP by Beta-secretase (BACE), which causes tau protein hyperphosphorylation and early-onset Alzheimer''s disease (AD). Here, we analyzed the effect of APP-sw in global gene expression using deep transcriptome sequencing technique. We found 283 genes were down-regulated and 348 genes were up-regulated in APP-sw expressing H4-sw cells compared to H4 wild-type cells from a total of approximately 74 million reads of 38 base pairs from each transcriptome. Two independent mechanisms such as kinase and phosphatase signaling cascades leading hyperphosphorylation of tau protein were regulated by the expression of APP-sw. Expressions of catalytic subunit as well as several regulatory subunits of protein phosphatases 2A were decreased. In contrast, expressions of tau-phosphorylating glycogen synthase kinase 3ß (GSK-3ß), cyclin dependent kinase 5 (CDK5), and cAMP-dependent protein kinase A (PKA) catalytic subunit were increased. Moreover, the expression of AD-relatedAquaporin 1 and presenilin 2 expression was regulated by APP-sw. Taken together, we propose that the expression of APP-sw modulates global gene expression directed to AD pathogenesis.
PubMed ID
No associated PubMed ID
Publication Title
No associated publication
Total Samples
4
Submitter’s Institution
Authors
No associated authors

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Processing Information
Additional Metadata
No rows found
Loading...