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Accession IconSRP056078

Impact of Tcf1 and Lef1 deficiency on follicular helper T (Tfh) cells

Organism Icon Mus musculus
Sample Icon 4 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Comparison of transcriptome between control and Tcf1/Lef1-deficient germinal center Tfh (GC-Tfh) cells Overall design: Control mice or those are deficient for Tcf1 and Lef1 transcription factors were infected with vaccinia virus. On day 8 after infection, the splenocytes were isolated and surface stained to identify CD44-high, CD62L-low, GFP+, CD4+ T cells. From these cells, PD-1+, CXCR5+ GC-Tfh cells were sorted for RNAseq analysis
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4
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