github link
Accession IconSRP052226

Zea mays subsp. mays Transcriptome or Gene expression

Organism Icon Zea mays
Sample Icon 15 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000, Illumina HiSeq 1500

Submitter Supplied Information

Description
DNA methylation is a stable modification of chromatin that can contribute to epigenetic variation through the regulation of genes or transposons. The genome-wide context-specific differential DNA methylation patterns and their influence on gene expression have rarely been investigated in crop species. Profiling of DNA methylation in five maize (Zea mays) inbred lines found that while DNA methylation levels for >99% of the analyzed genomic regions are similar there are still 5,000 to 20,000 context-specific differentially methylated regions (DMRs) between any two genotypes. The analysis of identical-by-state genomic regions that have limited genetic variation provided evidence that DMRs can occur without local sequence variation but they are 50% less common than in regions with genetic variation. Characterization of the sequence-specificity of DMRs, location of DMRs relative to genes and transposons and patterns of DNA methylation in regions flanking DMRs reveals distinct subset of DMRs. RNAseq profiling of the same tissue revealed that only ~20% of genes with qualitative (on-off) differences in gene expression are associated with DMRs and there is little evidence for association of DMRs with genes that show quantitative differences in gene expression. We also identify a set of genes that may represent cryptic information that is silenced by DNA methylation in the reference B73 genome. Many of these genes exhibit natural variation in other genotypes suggesting the potential for selection to act upon existing epigenetic natural variation. This study provides insights into the origin and influences of DMRs in a crop species with a complex genome organization.
PubMed ID
No associated PubMed ID
Publication Title
No associated publication
Total Samples
15
Submitter’s Institution
Authors
No associated authors

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Specimen part
Processing Information
Additional Metadata
No rows found
Loading...