Hepatic transcriptome changes in testosterone-deficient pigs fed a high-fat diet

To gain insight into the role of testosterone in modulating hepatic fat accumulation, we collected liver tissues from high fat diet-fed intact male pigs, castrated male pigs, and castrated male pigs with testosterone replacement. RNA-Seq was employed to profile hepatic gene expression in pigs with different testosterone levels. Overall design: Liver mRNA profiles of intact male pigs fed a HFC diet, castrated male pigs fed a HFC diet, and castrated male pigs treated with testosterone fed a HFC diet were generated by deep sequencing, using Illumina HiSeq 2000.

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Cai Z, Jiang X, Pan Y, Chen L, Zhang L, Zhu K, Cai Y, Ling Y, Chen F, Xu X, Chen M